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Studies on the Quality Standard of Pai shi Mixture

¡¡¡¡Abstract¡¡¡¡Pai shi mixture is a newly developed herbal prepara tion composed of Glechoma longituba (Nakai) Kupr. as the main compo nent supplement ed by Achyranthes bidentata, Plantago asiatica and several other minor ingredients. It was proposed for the treatment of urinary infection, hem at uria and urinary calculi. The object of the present study is to establish a sta n dard for the quality control of the product. The presence of flavonoids and sap o nins were varified by specific qualitative identification. G. longitub a and A. bidentata were distinguishing by TLC. Content o f total flavonoids was determined b y colorimetry as described in the 1995 edition of Chinese Pharm acopoeia. The plot of concentration against absorption gave a straight line ([ W TBX¡½r=0.999 6) over the range of 0.008¡«0.048 mg/mL. The averag e recovery of low, middle and high concentrations were 100.39%, 102.36% and 10 1.17% respect ively. The method proved to be simple, rapid and reproducible.

¡¡¡¡Key words¡¡¡¡Pai shi Mixture¡¡total flavonoid¡¡colorimetry¡¡Glechoma longituba (Nakai) Kupr.

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